How to Make Enzyme Cleaner: Easy Homemade Recipe (2024)

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Beat stubborn stains and pet odors with this DIY cleaning solution

Co-authored byand Hunter Rising

Last Updated: June 22, 2023Fact Checked

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  • Ingredients
  • |
  • Mixing the Cleaner
  • |
  • Fermenting the Cleaner
  • |
  • Using DIY Enzyme Cleaner
  • |
  • Video
  • |
  • Expert Interview
  • |
  • |
  • Things You'll Need

Do you want the stain-fighting power of an enzyme cleaner without buying an expensive bottle from the store? Well making your own enzymatic cleaner at home is a breeze with a few simple ingredients from your kitchen. With your homemade spray, you can easily get rid of stains left by blood, grass, sweat, urine, and so much more. We’ll walk you through everything you need to know about mixing and fermenting your own cleaner so you can start using it.

Things You Should Know

  • Put chopped citrus peels, lukewarm water, brown sugar, and yeast in a plastic bottle.
  • Let the enzyme cleaner ferment in a warm room for at least 1 month. Shake the bottle and open it daily to release gas buildup.
  • Strain out the citrus peels and transfer your enzyme cleaner into an airtight container.
  • ​​Use the cleaner diluted with water as an all-purpose cleaner or apply it undiluted to remove stains.

Part 1

Part 1 of 3:

Mixing the Cleaner

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  1. 1

    Wash and chop 2 cups (300 g) of fresh citrus peel. Peels or rinds from lemons, limes, grapefruit, oranges, and pineapples all work great for making an enzyme cleaner. Use a peeler to take the peel off the fruit, and rinse it under running water. Scrub the outside with a vegetable brush to remove any dirt, and pat the peels dry with a clean towel. Then, carefully chop the peels into 12in (1.3cm) cubes with a sharp knife.[1]

    • Avoid using peels that look dried out or rotted. Dried peels won’t contain enough citrus oil for cleaning, and rotten ones will cause the mixture to mold.
  2. How to Make Enzyme Cleaner: Easy Homemade Recipe (5)

    2

    Put the peels, sugar, water, and yeast in a large bottle. Insert a wide-mouthed funnel into the mouth of a clean 2L (0.53USgal) plastic soda bottle. Add the chunks of citrus peel in one handful at a time. Then, add ½ cup (100 g) of brown sugar, 1 teaspoon (3 g) of yeast, and 4¼ cups (1 L) of lukewarm water. Screw the bottle cap on tightly, and shake the bottle until all the sugar dissolves.[2]

    • Alternatively, use a blender to combine all of the ingredients until they have an even consistency.[3]

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  3. 3

    Label and date the bottle of cleaner. Use a marker to write “enzyme cleaner” clearly on the bottle so no one mistakes it for a drink. Then, include the date you added everything to the container so it’s easier to track how long you’ve let it ferment.[4]

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Part 2

Part 2 of 3:

Fermenting the Cleaner

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  1. 1

    Store the bottle in a warm spot. Keep the bottle on your countertop away from direct sunlight or on top of your refrigerator while the yeast and peels ferment. After about 1 month, your cleaner is strong enough to start using around your home.[5]

  2. How to Make Enzyme Cleaner: Easy Homemade Recipe (9)

    2

    Unscrew the cap to vent gas buildup 2–3 times a day. As the yeast eats the sugar, it produces alcohol and carbon dioxide that build up inside the bottle. Take the top off the bottle and gently squeeze the excess gas out before tightening the cap again. Screw the cap back on.[6]

    • Vent the bottle a few times daily for the first 2 weeks. After that, open the bottle every other day instead.
    • If you forget to vent the bottle, gas could build up and cause the bottle to break.
    • Make sure the cap is screwed on tight when you aren’t venting the bottle since yeast needs an oxygen-free environment to ferment properly. Oxygen will also allow bacteria and mold to grow in the mixture.
  3. How to Make Enzyme Cleaner: Easy Homemade Recipe (10)

    3

    Shake the bottle every day to mix the cleaner. Over time, the citrus peels will sink to the bottom. After you finish venting the gasses for the day, screw the lid back on and shake the mixture gently to stir up the contents.[7]

    • It’s normal for white build-up to form inside of the container. That just means your citrus peels are fermenting properly.
  4. 4

    Strain the fruit peels out of the cleaner after 1 month of fermenting. Wait until the mixture is opaque, which means it’s ready to use and strain. Put a strainer over a large bowl. Then, pour the mixture through the strainer to separate the solid peels from the actual cleaning solution.[8]

    • Throw away the citrus peels once you’re finished straining them.
    • If you blended the fruit peels into the water, make sure you use a fine-mesh strainer so none of the peel pieces are left in the liquid.
    • Leave the enzyme cleaner in the bottle for up to 3 months for an even stronger solution.
  5. 5

    Pour your cleaner back in the bottle to store it. Rinse out the bottle you used to ferment the cleaner and put a funnel in the spout. Pour your homemade enzymatic cleaner back into the bottle. Seal the cap on the bottle so it’s completely airtight.

    • Exposing the mixture to oxygen causes it to lose its potency, and it won’t clean as effectively.
    • To have some cleaner easily on hand, put some in a spray bottle and keep the rest in a larger bottle near your other cleaners.
    • Your enzyme cleaner will keep indefinitely as long as you keep the bottle sealed in between uses. If you notice any black mold growth in the liquid, toss it out.
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Part 3

Part 3 of 3:

Using DIY Enzyme Cleaner

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  1. 1

    Mix 1 part cleaner with 20 parts water for delicate jobs. Mix your cleaner and water in a spray bottle, and shake it up until they’re combined. Try this mixture for washing cars, cleaning fruits and vegetables, mopping floors, or for other jobs around the house that don’t require a super-powered cleaner.[9]

  2. How to Make Enzyme Cleaner: Easy Homemade Recipe (15)

    2

    Make an all-purpose cleaner with 1 part cleaner and 4 parts water. Combine the ingredients in a spray bottle so it’s easier to apply.[10] Spray the surface that you’re cleaning with the homemade enzyme cleaner and let it sit for a few minutes before wiping it up.[11]

    • Use your all-purpose cleaner for your bathrooms, carpets, kitchens, minor stains, and anything else you need to clean around your home.
    • For an even stronger cleaner, try mixing the enzyme solution with vinegar instead.
  3. 3

    Use the cleaner undiluted for the toughest jobs. For cleaning pet urine, stubborn stains, caked-on grime, and built-up dirt, apply the homemade enzyme cleaner directly to the affected surface. Let the cleaner sit for a couple minutes, and then wipe the area with a damp sponge or cloth.[12]

    • Enzyme cleaners are great for cutting grease around your kitchen and garage.
    • Try undiluted cleaner for removing scale and lime buildup on things like dishwashers, kettles, shower heads, and other appliances.
  4. 4

    Add 12 cup (120ml) of cleaner to your laundry to fight set-in stains. Add the enzyme cleaner to your load of laundry along with your regular detergent. Set and run your washing machine to the normal cycle and let it run for added power at breaking apart dirt and stains.[13]

    • Homemade enzyme cleaner also works as a fabric softener.
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Community Q&A

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  • Question

    Can I put this in a spray bottle and use it to spray in a nursing home?

    How to Make Enzyme Cleaner: Easy Homemade Recipe (18)

    Community Answer

    You must use only FDA-approved products purchased only through the nursing home's suppliers (who are licensed and bonded). This is a legal issue.

    Thanks! We're glad this was helpful.
    Thank you for your feedback.
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    Not Helpful 11Helpful 27

  • Question

    Can I shorten the time to make the cleaner by using a slow cooker?

    How to Make Enzyme Cleaner: Easy Homemade Recipe (19)

    Community Answer

    No, I would not recommend trying to alter the methods shown here.

    Thanks! We're glad this was helpful.
    Thank you for your feedback.
    If wikiHow has helped you, please consider a small contribution to support us in helping more readers like you. We’re committed to providing the world with free how-to resources, and even $1 helps us in our mission.Support wikiHow

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  • Question

    Do you need to add orange peels if you plan to just use it in your homemade laundry detergent? The goal is to be unscented.

    How to Make Enzyme Cleaner: Easy Homemade Recipe (20)

    Alkemi Clean Greensboro

    Community Answer

    Without the d-limonene found in the citrus, your mixture's cleaning ability is likely to be reduced. You can always try it and see if it works for you.

    Thanks! We're glad this was helpful.
    Thank you for your feedback.
    If wikiHow has helped you, please consider a small contribution to support us in helping more readers like you. We’re committed to providing the world with free how-to resources, and even $1 helps us in our mission.Support wikiHow

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      Ingredients

      • ½ cup (100 g) of brown sugar
      • 1 teaspoon (3 g) of yeast
      • 4¼ cups (1 L) of lukewarm water
      • 2 cups (300 g) of fresh citrus peel

      Things You'll Need

      • Vegetable brush
      • Knife
      • Funnel
      • Pop bottle
      • Strainer
      • Airtight bottle or jar
      • Spray bottle

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      Expert Interview

      Thanks for reading our article! If you'd like to learn more about making an enzyme cleaner, check out our in-depth interview with .

      More References (4)

      About This Article

      How to Make Enzyme Cleaner: Easy Homemade Recipe (33)

      Co-authored by:

      House Cleaning Professionals

      This article was co-authored by and by wikiHow staff writer, Hunter Rising. Claudia and Angelo Zimmermann are the founders of Everneat, an Eco-Friendly Cleaning Service based in New York City and in Connecticut. They are also the founders of Clean Code, a DIY 100% natural cleaning product line. This article has been viewed 713,884 times.

      11 votes - 100%

      Co-authors: 13

      Updated: June 22, 2023

      Views:713,884

      Categories: Stain Removal

      Article SummaryX

      To easily make enzyme cleaner, cut the peel of a citrus fruit into half-inch cubes. Insert a funnel into a large bottle, like a soda bottle, and pour ½ of a cup of sugar, 1 teaspoon of yeast, 4.5 cups of lukewarm water, and the cubed citrus peel into the bottle. Then, screw the cap onto the bottle and shake it vigorously until the sugar is dissolved. Place the bottle somewhere warm to ferment, and unscrew the cap daily to release built up gases. After two weeks, strain the mixture and use it as needed! For tips on properly diluting enzyme cleaner, read on!

      Did this summary help you?

      In other languages

      Deutsch:Enzymreiniger herstellen

      Español:hacer un limpiador con enzimas

      Русский:сделать ферментный очиститель

      Français:fabriquer un produit nettoyant enzymatique

      Bahasa Indonesia:Membuat Pembersih Enzimatik

      Nederlands:Een enzymreiniger maken

      العربية:صناعة المنظفات الإنزيمية

      中文:制作含酶清洁剂

      हिन्दी:एंजाइम क्लीनर तैयार करें (Make Enzyme Cleaner)

      한국어:효소 세척제 만드는 방법

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      Reader Success Stories

      • How to Make Enzyme Cleaner: Easy Homemade Recipe (34)

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        Apr 30, 2017

        "I didn't realize that you could use this in the washing machine! It took out a mildew odor from my front..." more

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      How to Make Enzyme Cleaner: Easy Homemade Recipe (2024)

      FAQs

      How to Make Enzyme Cleaner: Easy Homemade Recipe? ›

      Put chopped citrus peels, lukewarm water, brown sugar, and yeast in a plastic bottle. Let the enzyme cleaner ferment in a warm room for at least 1 month. Shake the bottle and open it daily to release gas buildup. Strain out the citrus peels and transfer your enzyme cleaner into an airtight container.

      How to make your own enzyme cleaner? ›

      The ratio of making enzyme cleaner is:
      1. 1 part brown sugar.
      2. 3 parts fruit scraps.
      3. 10 parts plain water. Some tips on making enzyme cleaner: Any fruit / veggie scraps would work, but citrus peels are one of the most fragrant options for your cleaner.
      Aug 3, 2017

      How to make enzyme solution? ›

      Steps to Make Bio-Enzyme Liquid for Plants
      1. Collect fruit/vegetable peels or waste from your kitchen.
      2. Chop the waste into small pieces.
      3. Take an empty plastic bottle or container.
      4. Add the kitchen waste and water in the container in equal parts.
      5. Add some jaggery to the solution (approx 50 grams in a 2-liter bottle).

      What is a substitute for enzyme cleaner? ›

      White Vinegar & Water Solution by wikiHow

      If you are looking for something almost entirely hassle-free, look no further than this very basic homemade enzyme cleaner made of two simple ingredients—white vinegar and water.

      What is a household enzyme cleaner? ›

      An enzyme cleaner is any cleaning agent that uses biological methods to digest organic waste, stains, molds, or odor.

      Does vinegar remove enzymes? ›

      Is vinegar/lemon an enzyme cleaner? No, both lemon and vinegar solutions are acidic cleaners that work by dissolving greasy, sticky, and similar soils from a surface and allowing water to wash them away.

      How are enzymes made simple? ›

      Enzymes are proteins comprised of amino acids linked together in one or more polypeptide chains. This sequence of amino acids in a polypeptide chain is called the primary structure. This, in turn, determines the three-dimensional structure of the enzyme, including the shape of the active site.

      How to create an enzyme? ›

      Enzymes are made from amino acids, and they are proteins. When an enzyme is formed, it is made by stringing together between 100 and 1,000 amino acids in a very specific and unique order. The chain of amino acids then folds into a unique shape.

      What is an enzyme ingredient? ›

      Enzymes are naturally-occurring proteins that enhance biochemical reactions. They can be obtained by extraction from plants or animals or by fermentation from micro-organisms. They are normally added to perform a technological function in the manufacture, processing, preparation and treatment of a wide range of foods.

      What household products work with enzymes? ›

      Enzymes are primary active ingredients in products such as powder and liquid detergents, stain removers, laundry pre-spotters, automatic dishwashing detergents, and industrial/medical cleaning products to name a few.

      How to make enzyme soap? ›

      The soap is prepared from, by weight, 20-50% of olive oil, 10-30% of coconut oil, 10-30% of palm oil, 5-11% of sodium hydroxide, 0.1-4% of honey, 8-30% of enzymes and 4.5-15% of water.

      Is OxiClean an enzyme cleaner? ›

      OxiClean™ Max Force™ Spray is an enzyme-based stain remover with 5-in-1 power that breaks down and removes numerous stains during the pretreatment process. It works to surround and loosen even the toughest set-in stains that have lingered on your clothes.

      What is the difference between enzyme cleaner and regular cleaner? ›

      So, one of the main differences between regular cleaners and enzymatic cleaners is that enzymatic cleaners perform residual cleaning, meaning they continue to clean once the product has been applied.

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